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. 2019 Mar 6;19:38. doi: 10.1186/s12876-019-0957-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of the membranous receptors Fzd1–10 along Barrett’s sequence. Expression of the receptors Fzd1–10 (a-j) were analyzed in EPC-1 and EPC-2, CP-A, CP-B, OE33 and OE19 cells by quantitative Real time RT-PCR. Highest expression levels of Fzd1 (a) and Fzd2 (b) were detected in CP-B. The receptors Fzd3 (c), Fzd4 (d) and Fzd5 (e) were strongly expressed in OE19. Overall, the expression of Fzd6 (f) and Fzd8 (h) were marginal and varied during Barrett’s sequence. The highest expression of Fzd7 (g) was found in OE33. The epithelial cell line EPC-1 expressed the highest levels of Fzd9 (i) and Fzd10 (j). Normalization was done with β-Actin. Values are shown as mean ± S.E.M. (One-way-ANOVA with Bonferroni correction, * - p < 0.05, **- p < 0.01 compared to EPC-1)