Table 2.
Antibodies
| Dilution | Company | Catalogue number | |
|---|---|---|---|
| Primary antibodies | |||
| pGSK3β-Ser9 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 5558 |
| pβ-catenin-Ser552 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 5651 |
| pAkt-Ser473 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 4060 |
| GSK3β | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 12456 |
| β-catenin | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 8480 |
| Akt | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 4691 |
| β-Actin | 1:2500 (0.5% LFM/PBS) | Sigma-Aldrich, Taufkirchen, Germany | A1978 |
| Secondary antibodies | |||
| Goat anti-rabbit | 1:7500 (0.5% LFM) | Jackson Immuno Research, Suffolk, UK | 111–035-045 |
| Goat anti-mouse | 1:7500 (0.5% LFM). | Jackson Immuno Research, Suffolk, UK | 115–035-068 |
BSA: bovine serum albumin; LFM: low fat milk; PBS: phosphate buffered saline