Table 5.

Selected RCM reaction with hCAII-based artificial metathase ArM 2.

entrya	hCAIIb	MCln (mol/L)	pH	TON
1 2 3 4 5 6 7 8 9	– WT – – WT – WT L198H L198H	MgCl2 (0.1) MgCl2 (0.1) – MgCl2 (0.5) MgCl2 (0.5) NaCl (0.154) NaCl (0.154) NaCl (0.154) –	6.0 6.0 7.0 5.0 5.0 7.0 7.0 7.0 7.0	48 ± 0.8 45 ± 2.0 23 ± 2.1 85 ± 1.0 78 ± 2.5 32 ± 2.0 21 ± 1.8 28 ± 0.6 22 ± 0.1

^aReaction conditions: [21]: 1 mM, [61]: 10 μM, [hCA II]: 12 μM, V_tot: 200 μL (DMSO 10%), 37 °C. Reactions carried out in triplicate. ^bWT = wild-type.