Table 6.

Selected RCM results obtained with artificial metathase ArM 1 using purified Sav samples.

entrya	substrate	catalyst (%)	proteinb	TON
1	62	60	–	1.1
2	62	60	Sav	1.7
3	62	60	Sav_mut5*	4.4
4	64	60	–	180 ± 4c
5	64	60	Sav	430 ± 3c
6	64	60	Sav_mut5*	650 ± 35c
7d	65	60	–	30 ± 1
8d	65	60	Sav	52 ± 2
9d	65	60	Sav_mut5*	90 ± 3

^aReaction conditions: 100 mM acetate buffer, 0.5 M MgCl₂, pH 3.6, [catalyst] = 50 µM, 16 h at 37 °C and 200 rpm. ^bSav_mut^5* = Sav V47A/N49K/T114Q/A119G/K121R. ^cTON determined by ¹H NMR. ^d[Substrate] = 20 mM; TON determined by UPLC–MS analysis.