Table 1.
Screening of hydrolases and conditions towards a model substrate.a
 | |||||||||
| entry | hydrolase | MeCN 5% | DMSO 5% | toluene 5% | MTBE 5% | ||||
| Tris·HCl | KPi | Tris·HCl | KPi | Tris·HCl | KPi | Tris·HCl | KPi | ||
| 1 | CAL-A | <1 | <1 | <1 | 3 | 1 | 1 | 2 | 1 |
| 2 | CAL-B | 93 | 89 | 90 (99)b | >99 | 97 | 97 | >99 | 93 |
| 3 | PSL-IM | 7 | 14 | 6 | 32 | 16 | 16 | 10 | 13 |
| 4 | PPL | 24 | 26 | 20 | 90 | 9 | 41 | 25 | 29 |
| 5 | CRL | 26 | 3 | 3 | 19 | 7 | 7 | 20 | 26 |
| 6 | Lypozyme TL-IM | 1 | <1 | <1 | 2 | 2 | 2 | 5 | 4 |
| 7 | Lypozyme RM | 2 | 2 | <1 | 5 | 2 | 3 | 2 | 2 |
| 8 | – | <1 | <1 | <1 | <1 | <1 | <1 | <1 | <1 |
aReaction conditions: 3 mg of 1a (30 mM) and 3 mg of enzyme, cosolvent 5% v/v, final volume 500 μL, 12 h; conversion was determined by GC-FID % relative area; b1.25 mg of 1a (final conc. 12 mM) and 2 mg of enzyme.