Figure 12.

Increased mast cell (MC) accumulation in newborn (P₀) Adtrp ^−/− mice. A, Transmission electron microscopy of the tail from wild‐type (WT) and Adtrp ^−/− pups. Yellow asterisks: MC accumulation. Black arrows: degranulated MC. Blue arrow: extravasated red blood cells. Red asterisks: decreased extracellular matrix density and enhanced edema. Bars, 10 μm. B, Bright‐field microscopy on whole‐mount diaphragms stained with Toluidine Blue showing MC (purple). Histogram: quantification and statistics (mean±SEM by unpaired t test) of MC numbers in the tail and diaphragm of P₀ pups (6 each). Markers are numbers of MCs counted in n fields of view (FOV) from at least 5 images/condition. ***P<0.001; ****P<0.0001. C, Double immunofluorescence with anti‐MC Tryptase (Cy3, red) and anti‐CD31 (FITC, green) IgGs, and whole‐mount confocal microscopy in cephalic skin of WT and Adtrp ^−/− newborn mice. Bars, 50 μm. BV indicates blood vessel; IgG, immunoglobulin G.