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. 2018 Dec 6;33(3):3887–3900. doi: 10.1096/fj.201800981R

Figure 7.

Figure 7

PAFAH2 attenuates CM- or TNF-α–induced lung injury in vitro and in vivo, respectively. A) HPAECs were transfected with an empty vector or full-length human PAFAH2 overexpressing plasmid (Addgene) for 24 h followed by treatment with CM (IL-1β, TNF-α and IFN-γ, 5 ng/ml each). TER was monitored for 20 h. The bar graph represents quantitative analysis of TER data after 15 h of cell stimulation; n = 4. *P < 0.05. B) Cells were pretreated with PAFAH2 inhibitor SB-480848 (1 µM) for 60 min followed by CM. Then, EC permeability was assessed by measuring TER for 16 h. The bar graph represents quantitative analysis of TER data after 15 h of cell stimulation; n = 4. *P < 0.05. C, D) C57/BL6 mice were exposed to TNF-α (10 μg/kg, i.t.) and PAFAH2 inhibitor SB-480848 (50 mg/kg, i.v.) concurrently, and an extra dose of SB-48048 (50 mg/kg, i.v.) was injected 5 h later. After 24 h, BAL was collected to analyze total cells (C) and protein content (D). Data are expressed as means ± sd; n = 3. *P < 0.05.