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. 2018 Dec 6;33(3):3601–3612. doi: 10.1096/fj.201801094RR

Figure 3.

Figure 3

Inhibition of miR-129-5p function restores NPC self-renewal and EGFR protein levels in LC NPCs. A–C) NPCs were cultured for 24 h in AC (70 μM) medium, transfected with control sc-miR-inh (B) or miR-129-5p (C) inhibitor (miR-129-Inh), along with pCAG-EGFP plasmid, and further cultured in AC (70 μM) (A) or LC (7 μM) conditions for another 48 h. EdU was added for 1 h at the end of the culture period. Transfection of miR-129-inh restores the proportion of proliferating EdU+ cells in LC conditions (C), compared to LC NPCs transfected with sc-miR-inh (B vs. C). D) Quantification of EdU+ cells among transfected NPCs (n = 4 per group). *P < 0.05, ***P < 0.001 by 1-way ANOVA. E) Quantification of EGFR protein levels was performed in EGFP-expressing cells coelectroporated with miR-129-inh vs. sc-miR-inh in LC E17 cortices by detection of immunofluorescence. **P < 0.01, by Mann-Whitney test.