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. 2018 Nov 7;33(3):3279–3290. doi: 10.1096/fj.201801114R

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© The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative histology images depicting cell pellet construct integration (circled red) within femoral defects stained with Alcian Blue (proteoglycans)/Sirius Red (collagen). A) Overview of pellet integration in all treatments and the no-pellet control sample. A dotted black line surrounds the defect area. Images underneath depict birefringence imaging of collagen fiber distribution (thick collagen fibers shown in orange-red; thin collagen fibers shown in green). B–D) Magnified images of the pellet implant of each treatment group; higher-magnification images of the same area below (dotted line). Femur drill defects containing remnants of HUVEC/HBMSC coculture pellet (B), HBMSC pellet (C), and HUVEC pellet (D). Arrows show collagen formation within and around the defect area. Scale bar, 100 µm.