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. 2018 Nov 7;33(3):3279–3290. doi: 10.1096/fj.201801114R

Figure 7.

Figure 7

A–F) vWF and CD31 (PECAM-1) immune expression in mono- and coculture pellet/defects. A) Representative images of vWF antigen were enhanced in all pellet treatments, particularly in implants containing HUVEC fractions (visible gaps in surrounding tissue at marrow cavity) as well as the surrounding tissue (arrows). B–G) Immunohistochemistry for CD31 expression in E18 chick femoral drill defects depicting different areas of 1 femur for each treatment. B–D) HUVEC/HBMSC coculture pellets (B), BMSC pellets (C), and HUVEC pellets (D); negative control (Neg C), where the primary antibody was omitted (inset). B, D) Dotted circle depicts defect area and below at higher magnification, with B showing distinct CD31 stained aggregates within the pellet (arrows). E–G) CD31-positive staining within the epiphysis of femurs. Note the tissue (G) infiltrated with CD31-positive cells along the periosteum of the femur. Arrows depict areas of immune-positive staining. E, epiphysis; M, marrow cavity. Scale bars, 100 µm.