Figure 5.

LIN28 reduces the total number of astrocytes at 21 DPE in the striatum. A) Representative ×40 micrographs showing GFP (green)/GFAP⁺ (red) astrocytes in the striatum of control (upper) and LIN28 (lower) at 21 DPE. Original scale bars, 50 μM. B) Bar graph displaying the average percent of GFP⁺ cells that are positive astrocytes in the striatum bordering the SVZ at 21 DPE; n = 12 slices (control), n = 15 slices (LIN28::GFP). C) The average number of GFP⁺ astrocytes in the striatum per slice at 21 DPE; n = 7 slices. D) Bar chart depicting the average number of GFP⁺ astrocytes per area (square millimeters) at 21 DPE; n = 7 slices. B–D) Data are presented as means ± sem. *P ≤ 0.05, ***P ≤ 0.005 vs. control, Student’s t test.