Figure 6.

Intracellular Aβ can be cleared by restoring metalloprotease activity. Cells were incubated with inhibitors for 48 h, followed by continued incubation or exchange to conditioned medium without inhibitors (washed). A, B) After washing out the inhibitors, intracellular levels of Triton X-100–soluble (A) and Triton X-100–insoluble (RIPA extractable) (B) Aβ40 returned to control levels (indicated by the dotted line). C) Intracellular levels of RIPA-extractable oAβ were completely undetectable in control cells and nearly undetectable in drug-treated cells after the washout period. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.