β-AR signaling regulates macrophage functions through changes in actin organization. A) Phagocytosis is measured in the presence of actin polymerization inhibitor (2 µM cytochalasin D), Arp2/3 inhibitor (50 µM CK666), or formin inhibitor (20 µM SMIFH2), whereas β-AR signaling is activated with 100 nM isoproterenol for 24 h. Statistical significance indicated by asterisks shows comparisons to vehicle control unless indicated. One-way ANOVA test with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001. B) Quantitative analysis of chemotaxis after 24 h of drug treatment. Statistical significance indicated by asterisks shows comparisons to vehicle control unless indicated. One-way ANOVA test with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001. N.s., not significant. Results represent data from at least 3 independent experiments (N = 3).