Figure 6.

Thy1 is hypermethylated in a time-dependent manner during adipocyte differentiation and attenuated by 5-aza-dC. 3T3-L1 cells were pretreated for 24 h with either DMSO or 5-aza-dC, along with continuous daily treatment, and then harvested corresponding to days with ACT (d 0, no ACT). A) Average percentage of DNA methylation of Thy1-CGI1 showed an increase in methylation in normal adipogenesis differentiation process, whereas treatment with the methylation inhibitor 5-aza-dC resulted in a decrease in overall methylation. B, C) Individual CpG position sites were measured across Thy1-CGI1, and d 0 showed no relevant changes, whereas d 6 had a notable decrease in methylation when treated with 5-aza-dC. *P < 0.05.