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. 2018 Nov 19;33(3):3636–3646. doi: 10.1096/fj.201801496R

Figure 4.

Figure 4

Effect of NES-mutation on SK2 translocation and lipotoxicity in β-cells. INS-1 β-cells were transfected with Flag-tagged WT, NES-mutant SK2, or a control empty vector (EV) followed by treatment with PA (400 μM) or control vehicle (Veh) for 12 h. A) Treated cells were fixed and incubated with antibodies to the Flag epitope followed by the secondary antibodies conjugated with AlexaFluor (green) and DAPI (blue). Photomicrographs were taken of individual fields. Original magnification, ×40. B, C) Western blotting (B) and flow cytometry–based apoptosis (C) were conducted as detailed in Fig. 1. Data are expressed as mean ± sd (n = 3). NS, not significant. *P < 0.001.