Figure 1.

Compound 011 specifically inhibits growth of MscL expressing bacteria. A) Minimal inhibitory concentration curves are shown for compound 120 (green) and 011 (red). Growth of the E. coli strain MJF612 (ΔmscL, ΔmscS, ΔmscK, and ΔybdG) carrying an empty plasmid (black) or expressing MscS (open squares) or Eco-MscL (solid squares) was measured as the OD₆₀₀ of the cultures. Values are expressed as the percentage decrease in growth in the presence of compound 120 or 011, relative to the nontreated samples (n = 3–8). B) Representative traces showing the effects on MscL channel activity of compounds 120 (upper traces) and 011 (lower traces). The schemes on the right illustrate 2 different configurations where the patch is presented with the compound from the periplasmic side (pipette) of the cytoplasmic side (bath). Channel activity was recorded by patch clamp experiments of native membranes, in a single patch before (left traces) and after (right traces) treatment with the compounds, at the same indicated pressure.