Figure 2.

The mode of action compound 011. A) Minimal inhibitory concentration curves are shown for compounds 120 or 011 in cultures of the sulfonamide resistant strain PB121 (open markers) and its parental MJF612 strain (closed markers). Bacterial cultures carrying an empty plasmid control (black) or expressing Eco-MscL, were treated with compound 120 (green) or compound 011 (red). Values represent the percentage decrease in growth (OD₆₀₀) vs. nontreated. B) Minimal inhibitory concentration curves are shown for the MJF612 strain treated with compound 011 or 011A. Note that 011A is similar to 011 but lacking the sulfonamide group. Bacterial cultures carrying an empty plasmid (control) are shown in black, treated with 011 (closed squares) or 011A (open squares); cultures expressing Eco MscL treated with 011 (red) or 011A (blue) are shown. Values represent the percentage decreased in growth in the presence of compound 011 or 011A vs. nontreated. C) The reduction in viability of stationary cultures treated with 011 (red) or 011A (blue), expressed as the percent reduction of colony forming units (CFUs) vs. no treatment (n = 3–5). ***P < 0.0005, ****P < 0.00005, Eco WT vs. empty plasmid (2-tailed, 2-sample homoscedastic t test).