Figure 2.

DMT1 expression and iron uptake are elevated in the duodenum of diabetic mice. A) The mRNA expression of iron metabolism genes in control (Cont) and diabetic (DM) mouse duodenum was measured by qRT-PCR. IRE, DMT1-IRE; after IRE and non-IRE, DMT1–non-IRE. *P < 0.01, **P < 0.05 compared with Cont. n = 5. B) Representative images show the expression of HIF2α (green) with F-actin (red) by phalloidin and nuclei (blue) by DAPI in duodenal epithelial cells of Cont and DM mice. n = 5. Scale bar, 50 μm. C) Duodena dissected from Cont and DM mice were exposed to 100 µM FeSO₄, and intracellular Fe²⁺ content was determined by labeling with 5 μM RhoNox-1 (red). Scale bar, 50 μm.