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. 2018 Nov 13;33(3):3549–3561. doi: 10.1096/fj.201801855R

TABLE 3.

Primers used for real-time PCR

Gene	Primer sequence, 5′–3′
Gene	Forward	Reverse
mDMT1-IRE	`TGGGTCTGTCTTTCCTGGAC`	`TGCAACAGCACATACTTGTGG`
mDMT1-non-IRE	`TGGGTCTGTCTTTCCTGGAC`	`GAACAAGCTCACCTCCGAAC`
mDMT1 (IRE and non-IRE)	`ATCGCCATCAATCTCCTGTC`	`AAACGTGAGGGCCATGATAG`
mDCytb	`CAATGGAAACGCCCTAGAGA`	`CGACATTTCACATGGTGGAC`
mFerroportin	`TGGAATTTGGTGTCCATGTG`	`TCAAGTTCACGGATGTTGGA`
mHephaestin	`CTGGCATGGAGACCATCTTT`	`AGCAATGAGCAACAGAAGCA`
mFerritin-H	`GCCAGAACTACCACCAGGAC`	`TGGTTCTGCAGCTTCATCAG`
mFerritin-L	`GGGCCTCCTACACCTACCTC`	`CTCCTGGGTTTTACCCCATT`
mHepcidin-1	`TTGCGATACCAATGCAGAAGA`	`GATGTGGCTCTAGGCTATGTT`
mβ-actin	`AGCCATGTACGTAGCCATCC`	`TCTCAGCTGTGGTGGTGAAG`
hDMT1 (all isoforms)	`CCAGGTACTCAAGGGCATGT`	`GTGCAATGCAGGATTCAATG`
hβ-actin	`GGACTTCGAGCAAGAGATGG`	`AGCACTGTGTTGGCGTACAG`

m, mouse; h, human.