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. 2018 Dec 3;33(3):3807–3824. doi: 10.1096/fj.201801910R

Figure 2.

Figure 2

Standard TIRF microscopy of LukS/F with hC5aR on HEK cells. A, left) TIRF image of hC5aR-mGFP on the surface of a HEK cell before addition of toxin; A, right) zoom-ins of yellow, dashed square (left) immediately following 2 min incubation with Alexa Fluor 647-labeled LukSK281CY113H [mS*(Alexa647)]. B) Equivalent images of the same cell of B after >15 min incubation with LukFK288C (mF). C, upper) TIRF image of colocalization of Alexa Fluor 594- and Alexa Fluor 647 mF* and mS* [mF*(Alexa594) and mS*(Alexa647)] with hC5aR-mGFP on HEK cells; C, lower) zoom-in of yellow, dashed square (upper) with colocalized foci indicated (arrows).