TABLE 2.
Stoichiometry and microscopic diffusion coefficient in live and fixed cells
| Type | Stoichiometry, mol (mean ± sd) | Foci (n) | Mobile foci D, μ2m/s (mean ± sd) | Immobile foci D, μ2m/s (mean ± sd) |
|---|---|---|---|---|
| Live cells | ||||
| C5a | 185 ± 224 | 5346 | 0.48 ± 0.43 | 0.03 ± 0.03 |
| C5a + mLukS* | 281 ± 213 | 8272 | 0.49 ± 0.42 | 0.02 ± 0.03 |
| C5a + mLukS* + mLukF | 291 ± 248 | 6605 | 0.46 ± 0.42 | 0.03 ± 0.03 |
| C5a + mLukS + mLukF* | 223 ± 202 | 4981 | 0.47 ± 0.40 | 0.02 ± 0.03 |
| mLukS* | 29 ± 22 | 999 | 0.44 ± 0.45 | 0.03 ± 0.03 |
| mLukS* + mLukF | 84 ± 89 | 841 | 0.34 ± 0.35 | 0.01 ± 0.02 |
| mLukS + mLukF* | 6 ± 4 | 557 | 0.40 ± 0.44 | 0.02 ± 0.03 |
| Colocalized C5a foci, n (/%) | C5a foci not colocalized, n (%) | Stoichiometry of colocalized C5a foci, mol (mean ± sd) | Stoichiometry of C5a foci that are not colocalized, mol (mean ± sd) | |
|---|---|---|---|---|
| Fixed cells | ||||
| C5a + mLukS* | 89 (32) | 193 (68) | 36 ± 26 | 35 ± 24 |
| C5a + mLukS* + mLukF | 84 (7) | 1079 (93) | 37 ± 25 | 32 ± 24 |
| C5a + mLukS + mLukF* | 59 (83) | 283 (17) | 26 ± 20 | 26 ± 20 |
| Colocalized Luk foci, n (%) | Luk foci not colocalized, n (%) | Stoichiometry of colocalized Luk foci, mol (mean ± sd) | Stoichiometry of Luk foci that are not colocalized, mol (mean ± sd) | |
|---|---|---|---|---|
| mLukS* | 88 (7) | 1198 (93) | 72 ± 47 | 61 ± 44 |
| mLukS* + mLukF | 86 (88) | 658 (12) | 121 ± 95 | 136 ± 94 |
| mLukS + mLukF* | 60 (5) | 1186 (95) | 20 ± 16 | 18 ± 15 |