Figure 4.

∆133p53β has a higher aggregation propensity because of the deletion of core domain residues. A) The SASA analyses of the p53 aggregation core fragment ²⁵¹ILTIITL²⁵⁷ in the 94-341p53β and Δ133p53β systems. B) Protein expression of p53 (94–312) and p53 (133–312). A total of 3 buffer conditions were tested for expression of both p53 fragments. A fraction of soluble p53 (94–312) can be obtained in the supernatant (S), but p53 (133–312) are all in pellet (P).