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. 2018 Dec 19;33(3):4473–4489. doi: 10.1096/fj.201802167R

Figure 1.

Figure 1

CR mediated differential translation in the liver. A) The experimental workflow. Mouse liver lysates were subjected to fractionation using sucrose gradient. Total RNA was extracted from polysome fractions and mRNA-seq was performed. B) The effect of CR on the P-mRNA abundance. P-mRNAs, whose abundance was increased (red), and P-mRNAs, whose abundance were decreased (black), in the liver polysomes in response to CR. C) Log ratio/mean average (MA plot) of log-fold change for mean of normalized read counts, every dot represents individual gene, gene for P-mRNAs with differential abundance between AL and CR samples are shown by red dots. D) Enriched pathways for P-mRNAs differentially abundant under CR treatment. Pathways were sorted in decreasing order of significance. Values on the pie chart represent the number of the P-mRNAs involved in the pathways. Cutoff value was set to P ≤ 0.01. E, F) Validation of mRNA-seq data: mRNA-seq data for Fmo3 and Serpina12 (n = 12) (E) and real-time quantitative PCR on RNA extracted from polysomal fraction for Fmo3 and Serpina12 (n = 12) (F). *P < 0.05.