Figure 5.

CR regulated the translation of enzymes regulating long-chain Acyl-CoA metabolism. A, B) P-mRNA abundance of FABPs. Fabp1 and Fabp2 in the liver polysomes of AL and CR animals. C, D) P-mRNA abundance for acyltransferases, Cpt1a and carnitine acetyltransferase (Crat) in the liver polysomes of AL and CR animals. E–G) P-mRNA abundance for Acyl-CoA dehydrogenase family proteins—Acadl, Acyl-CoA dehydrogenase, short/branch chain, and Acyl-CoA dehydrogenase, medium chain—in the liver polysomes of AL and CR animals. H, I) P-mRNA abundance for N-acyltransferases—Acnat1 and Acnat2—in the liver polysomes of AL and CR animals. J–L) P-mRNA abundance for Acot1, Acot3, and Acot4 in the liver polysomes of AL and CR animals. Light/dark bar at the top represents light and dark phases of the day; light is on at ZT0, and off at ZT12. Two biologic replicates for each time point of AL animals were compared to those of CR for differential P-mRNA abundance using Deseq2 software. Log₂ values for the fold change thus obtained were used to plot graphs (n = 2 for each time point for each diet).