Figure 2.

Diagram depicting the main steps of Iso-Seq read analysis. Raw Iso-Seq reads were processed to obtain Full-Length Non-Chimeric (FLNC) reads and clustered isoform reads (PCIRs). FLNC reads that did not cluster in any of the PCIRs were error-corrected using RNA-seq data (C-FLNC). The final dataset described in this study included PCIRs and C-FLNC reads. For each step, sequencing accuracy and predicted coding sequence (CDS) length distributions are reported.