Figure 4.

XO karyotype underlies preferential death of males in feminized mir-35-41(nDf50) animals. A) Schematic of cross. Males contain a GFP-marked nT1 balancer and an X-linked mCherry transgene which aid in distinguishing fem-3 genotype and number of X chromosomes in progeny. B) Percent of progeny from crosses in each category. Dead/arrested embryos and larvae were not scored for fluorescent markers, and thus are likely a mixture of genotypes. C) XO:XX ratios in populations of progeny from crosses. Corrected values assume that differences in XO:XX ratio in nT1+ and nT1- population in the control mir-35-41(nDf50); fem-3(wild type) cross are due to non-Mendelian segregation of the nT1 balancer.