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Localization of Pal2-GFP and Pal1-3xGFP in the absence of other endocytic factors. (A) Yeast strains expressing Pal2-GFP (WT; SL7455) and in ede1∆ (SL7505), syp1∆ (SL7405), yap1801/2∆ (SL7401), pal1∆ (SL7475), chc1∆ (SL7451). (B) Yeast strains expressing Pal1-3xGFP (WT; SL7335) and in ede1∆ (SL7502), syp1∆ (SL7579), yap1801/2∆ (SL7581), pal2∆ (SL7400), chc1∆ (SL7468). Images shown are from a single plane of a z-stack focused on the middle of the cell. Scale bar: 5μm.
