Figure 3.

Conservation of OLFML3 predicted amino acid sequence A. Amino acid sequence of the canine OLFML3 protein. Functional domains are highlighted: blue – signal peptide; pink – coiled coil domain; yellow – olfactomedin domain (Zeng et al. 2005; Rodríguez-Sánchez et al. 2013). Within the yellow region, bold lettering shows the core OLF region. Green shows the arginine at position 197. B. Conservation of arginine 197 (green) in OLFML3 of vertebrates. Arginine is found at the equivalent position in all mammal, bird and reptile species for which there is an annotated OLFML3 gene, and in coelacanth but not in other fish. The region around this arginine is also highly conserved.