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. 2019 Jan 10;9(3):749–754. doi: 10.1534/g3.118.200923

Figure 1.

Figure 1

Shep ERRM mutant is defective for RNA binding in vitro. A. Conservation of Shep E RRM domains compared to human and mouse orthologs. Consensus resides of RNP1 and RNP2 in RRM domains are shaded for Shep human and mouse orthologs. Residues mutated to alanine of the RNP1 domains are indicated as blue mutated to red. B. EMSA of wildtype Shep E vs. Shep ERRM mutant. 455nt ssRNA fragment of brm or 470nt ssRNA fragment of CG10555 was mixed with buffer, recombinant wildtype Shep E, or Shep ERRM mutant and run on a 6% GTG polyacrylamide gel.