Figure 4.

Wildtype Shep E but not Shep E^RRM mutant rescues developmental defects of shep mutant bursicon neurons. A-H. Homozygous but not heterozygous loss of shep (shep^BG00836) leads to smaller cell bodies and fewer projections. Expression of wildtype Shep but not the RRM mutant using a Gal4 driver inserted into the shep locus rescues these morphological defects. Bursicon neurons and their projections are visualized by immunostaining with anti-BURS. I. Soma sizes are quantified by Photoshop and compared by One-way ANOVA followed by Tukey HSD post hoc tests, with statistical threshold at 0.05, ** P < 0.01. Sample sizes are indicated in parentheses. J. Projections of bursicon neurons are quantified by Sholl analysis. Sample sizes are indicated in parentheses.