Table 2. Normalized RNAseq quantification of pre-MIR828, TAS4, and target MYBA6 and -MYBA7 mRNAs in berry skins of five field-grown red and white cultivars during development (datasets of Massonnet et al. 2017).
| Datasets/gene | Berry Developmental Stage | |||
|---|---|---|---|---|
| Pea-sized | Just prior veraison | Soft | Harvest | |
| Red cultivars | (reads per 100 Million*) | |||
| pre-MIR828^ | 1.1 | 0.0 | 0.2 | 0.0 |
| TAS4a^ | 31.4 | 16.5 | 6.0 | 0.4 |
| TAS4b^ | 0.3 | 0.2 | 0.0 | 0.0 |
| TAS4c& | 0.0 | 0.0 | 13.6 | 8.7 |
| MYBA6$ | 5.1 | 1.6 | 10.3 | 0.9 |
| MYBA7^ | 19.9 | 13.3 | 0.0 | 0.0 |
| pre-MIR166e reference^ | 26.5 | 20.2 | 13.2 | 7.8 |
| White cultivars | ||||
| pre-MIR828^ | 1.3 | 0.2 | 0.0 | 0.0 |
| TAS4a^ | 53.8 | 41.7 | 19.1 | 3.9 |
| TAS4b^ | 4.6 | 4.3 | 0.0 | 0.0 |
| TAS4c | 0.0 | 0.0 | 0.0 | 0.0 |
| MYBA6$ | 6.0 | 3.7 | 5.2 | 3.8 |
| MYBA7^ | 8.2 | 0.0 | 0.2 | 0.0 |
| pre-MIR166e reference^ | 23.0 | 14.4 | 5.8 | 3.9 |
96 nt reads mapping completely to amplicons quantified by qRT-PCR in Fig. S2, <= 4 mismatches. See Supplementary Table 7 for amplicon target details. Pre-vvi-MIR166e target sequence from miRBase22; TAS4b reads are for 105 bp amplicon plus 100 nts upstream and downstream because 96 nt read lengths precluded results.
The decreasing pre-MIR828, TAS4ab, MYBA7, and pre-MIR166e expressions as development progresses correlate with the derivative miR828*, TAS4 siRNA, MYBA7 mRNA, and mature miR166 abundances documented in field samples, Supplementary Table 3d and Supplementary Fig. S2.
&The increasing TAS4c expression as development progresses (bold) correlates with mRNA and derivative TAS4c tasiRNA 3′D4(-) abundances documented in field samples, Supplementary Table 3d and Fig. S2E.
The fluctuations of MYBA6 expressions at veraison and soft berry stages (italics) correlate with the derivative siRNA abundance documented in field samples, Supplementary Table 3d and Fig. S2F, left panel.