Figure 2.

In vitro and ex vivo modulation of ferritin heavy chain and MHC-I expression in mouse. (A) Immortalized Embryonic fibroblasts derived from NCOA4 WT and KO mice (MEFs) were surface stained for MHC class I molecules H2 K (b) (apex) and H2 D (b) (apex) and analyzed by flow cytometry. Data are representative of three independent experiments. (B) Cell surface levels of H2-K (b) (apex) and H2-D (b) (apex) were analyzed on freshly explanted splenocytes of NCOA4 KO mice compared to those of wt mice. White column represents wt (B6) mice while gray column corresponds to NCOA4 KO mice. Data are representative of three consecutive experiments. P-values were calculated using 2-tailed Unpaired Student t-test. ^*p < 0.05; ^** p < 0.01; ^*** p < 0.001.