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. 2019 Feb 26;10:224. doi: 10.3389/fimmu.2019.00224

Figure 4.

Figure 4

Iron and Ferritin Heavy Chain reduction is associated with NK cell susceptibility of established tumor cell lines and primary melanoma cells. (A,B) Representative cytotoxicity experiments in which two primary melanoma cell lines, Mel-30 and Mel-35, where treated overnight with DFO. Curves show the NK cell recognition of DFO treated cells (gray squares), and untreated cells (white squares). Columns show statistical analysis of three consecutive experiments at the effector:target ratio of 25:1 and 12:1, respectively. (C,D) Representative cytotoxicity experiments in which MM07m shFTH and MCF7 shFTH (gray squares), or scramble (white squares) were tested for their susceptibility to NK cell killing. Column show statistical analysis of five consecutive experiments at the effector:target ratio of 25:1 and 12:1, respectively. P-values were calculated using paired Student t-test (*p < 0.05; **p < 0.01; ***p < 0.001).