Figure 3.

Fold-change of KAR2 expression in the P. pastoris strain producing EcPGA and the non-producing control strain as obtained by qPCR analysis. The P. pastoris strains producing EcPGA (green) and the control non-production strain (black) were cultured under the same cultivation conditions (30°C, pH 5.5), maintaining the specific growth rate of biomass with methanol at 0.016 h⁻¹. All relative expression data are related to the expression of KAR2 in the non-producing control strain in the sample taken 3 h after induction.