Figure 7.

Specific fluorescence of the culture medium and cell lysates of the P. pastoris strain producing TlXynA, and the control strains. The P. pastoris strain producing TlXynA was cultured at 30°C and pH 5.5, maintaining the specific growth rate of biomass with methanol during the production phase at 0.016 h⁻¹. Fluorescence of the centrifuged culture medium and of the cell lysates were measured with a fluorimeter. Specific extracellular fluorescence (dark red) and specific intracellular fluorescence (light red) were calculated from the measured fluorescence (AU) and CDW (g) values. As controls, the specific extracellular fluorescence of the EcPGA-producing strain with no sfGFP production (gray), and the non-producing strain (black) are shown. The control strains were cultured under the same cultivation conditions as the TlXynA-producing strain.