Figure 8.

CaLB/biomass yield during cultivations of P. pastoris producing recombinant CaLB under different specific growth rates with methanol. The P. pastoris strain producing CaLB was cultured at 30°C and pH 5.5, maintaining the specific growth rate of biomass with methanol during the production phase at either 0.008 h⁻¹ (purple), 0.016 h⁻¹ (blue), or 0.032 h⁻¹ (brown). The secreted (dark purple/blue/brown circles) as well as the intracellular (light purple/blue/brown squares) CaLB/biomass yield were calculated.