Table 1. Crystallization.
Method | Vapor diffusion |
Plate type | Sitting drop |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 12 |
Buffer composition of protein solution | 20 mM Tris, 40 mM NaCl pH 8.0 |
Composition of reservoir solution | 22.5% saturated ammonium sulfate, 0.1 M sodium acetate pH 5.0 |
Volume and ratio of drop | 1100 µl (1:1) |
Volume of reservoir (ml) | 50 |