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. 2019 Feb 20;75(Pt 3):171–175. doi: 10.1107/S2053230X19000724

Table 1. Crystallization.

Method Vapor diffusion
Plate type Sitting drop
Temperature (K) 293
Protein concentration (mg ml−1) 12
Buffer composition of protein solution 20 mM Tris, 40 mM NaCl pH 8.0
Composition of reservoir solution 22.5% saturated ammonium sulfate, 0.1 M sodium acetate pH 5.0
Volume and ratio of drop 1100 µl (1:1)
Volume of reservoir (ml) 50