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. 2019 Feb 21;75(Pt 3):205–211. doi: 10.1107/S2053230X19000712

Table 1. Macromolecule-production information.

Source organism C. crocatus Cm c5
DNA source Genomic DNA
Forward primer CTTCCATGGCAGAGGAAGTCGCCGAG
Reverse primer CTTAAGCTTTCATCTCTCGGGCCACAACGT
Cloning vector pHis-SUMO-TEV
Expression vector pHis-SUMO-TEV
Expression host E. coli
Complete amino-acid sequence of the construct produced MGSSHHHHHHGSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGDIPTTENLYFQGAMAEEVAEIILPASTWILFFDASCSINSPAFWSTNDAVDRIWRLKIAHELVLLQVVLEGYFKVRCILRSSAPAFEMVNADVSELVSIVLPSGRLVACTTDEPTLNRHVLTVPPGRYRVLREWSVHEESKHYDVESAEAYPADEGPDGIITLWPER