FIG. 3.

Separation of polydispersed fluorescent nanoparticles. (a) Representative fluorescence micrographs of the separation channel before centrifugation filled with 0.1% v/v polydispersed fluorescent nanoparticles (500 nm in red and 200 nm in green) within the microfluidic chip. (b) Corresponding fluorescence micrographs after centrifugation for the polydispersed nanoparticles. The scale bar represents 100 μm. (c) Micrograph of the inner portion of the microfluidic channel under 20× optical magnification showed monodispersed nanoparticles, indicating that the larger nanoparticles have migrated to the outermost channel wall. (d) Fluorescence micrograph of the outer microchannel indicating a higher fluorescence intensity at the outermost channel wall. The scale bar represents 50 μm.