. 2018 Nov 28;12(6):064104. doi: 10.1063/1.5061922

TABLE II.

Observed cell behaviors and utility of different dimensioned micro-stencils.

Pattern diameter	Spread area	Predominant configuration	Application
Pattern diameter	Spread area	Predominant configuration	Cell motility	Cell-cell communication	Cell growth
20 μm	∼300 μm²	Singlets	No	No	No
30 μm	∼700 μm²	Doublets	No	Yes^a	Yes^a
40 μm	∼1250 μm²	Triplets	Yes	Yes	Yes^a
50 μm	∼2000 μm²	Clusters	Yes	Yes	Yes^a

^{^a}

These dimensions can be used for the respective applications when coupled with appropriate seeding densities. The combination of these factors allows for control over resulting cell configurations.