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Comparison between miRNA and DNA in ICP hybridization.
| miRNA (miRNA 21) | DNA15 | |
|---|---|---|
| MO concentration (capture probe) | 100 μM | 40 μM |
| MO capture probe | 5′ NH2-CAG TCA ACA TCA GTC TGA TAA GCT A 3′ | 5′ NH2-GTA GCT AAT GAT GTG GCA TCG GTT 3′ |
| Voltage during ICP hybridization | 15 V | 75 V |
| Heat applied to sample before experiment | 50 °C for 7 min | No heat |
| Plug stability | 1 h | 30 min |
| Concentration factor | 90× (Co = 100 nM) | 1000× (Co = 10 nM) |
| Concentration time | 30 min | 5 min |
| Limit of detection | 25 nM | 1 nM |
