KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-DR5 (Human) | Cell Signaling Technology | Cat#3696 |
| Anti-DR4 (Human) | Cell Signaling Technology | Cat#42533 |
| Anti-FOLR1 | Thermo Fisher | Cat#PA5–24186 |
| α-Tubulin Antibody | Cell Signaling Technology | Cat#2144 |
| Cleaved Caspase-3 Antibody | Cell Signaling Technology | Cat#9661 |
| Capase-3 Antibody | Cell Signaling Technology | Cat#9668 |
| GAPDH Antibody | Cell Signaling Technology | Cat#5174 |
| Caspase-8 Antibody | Santa Cruz Biotechnology | Cat#8CSP03 |
| E-Cadherin Antibody | Cell Signaling Technology | Cat#14472 |
| Anti-Rabbit-HRP antibody | Cell Signaling Technology | Cat#7074 |
| Anti-Mouse-HRP antibody | Cell Signaling Technology | Cat#7076 |
| Anti-DR5 (Mouse) antibody | Abcam | Cat#Ab8416 |
| Cleaved PARP antibody | Cell Signaling Technology | Cat#9953 |
| Commercial LK26 (anti-murine FOLR1) antibody | Abcam | Cat#Ab3361 |
| Commercial MD5–1 (anti-Murine DR5) antibody | Abcam | Cat#ab171248 |
| Anti-Human IgG1 HRP | Life Technologies | Ref#A10648 |
| Anti-GFP antibody | Santa-Cruz Biotechnology | Cat#sc-9996 |
| Mouse anti-human IgG1 Fc | Thermo Fisher | A10648 |
| Bacterial and Virus Strains | ||
| E. coli Stellar™ | F′, endA1, supE44, thi1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔM15, Δ(lacZYA-argF) U169, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, λ- | Clontech |
| BL21 Competent E. coli | NE Biolabs | C2530H |
| DH5α | Thermo Fisher | 18258012 |
| Biological Samples | ||
| PDX cell line: V584 | Dr. Chip Landen, | https://cancer.uvahealth.com/research/shared-resources/maps |
| PDX cell line: V565 | Dr. Chip Landen, | https://cancer.uvahealth.com/research/shared-resources/maps |
| PDX cell line: 111 | Dr. Chip Landen, | https://cancer.uvahealth.com/research/shared-resources/maps |
| PDX cell line: 135R | Dr. Chip Landen, | https://cancer.uvahealth.com/research/shared-resources/maps |
| Patient-derived xenografts (PDX) | UVA MAPS core/Tumor bank | https://cancer.uvahealth.com/research/shared-resources/maps |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Apo2L | R&D systems | Cat#375-TL |
| Recombinant HER2 | R&D systems | 1129-ER-050 |
| Recombinant IgG4-Fc-DR5 (rDR5) | This paper | |
| Recombinant IgG4-Fc-FOLR1 (rFOLR1) | This paper | |
| Cisplatin | Sigma | NC0837572 |
| EZ-Link Sulfo-NHS-SS-Biotin | Thermo Fisher | 21331 |
| Critical Commercial Assays | ||
| Endpoint Chromogenic LAL endotoxin assay kit | Lonza | 50–648U |
| AlamarBlue Cell viability reagent | Thermo Fisher | DAL1100 |
| MTT reagent | Thermo Fisher | |
| AST reagent | Pointe Scientific | 23-666-1221 |
| EnzyChrom ALT Assay Kit | Bioassay Systems | EASTR-100 |
| Deposited Data | ||
| doi:10.17632/tgnzd6m8y5.1 | This Paper | Shivange et al., |
| Experimental Models: Cell Lines | ||
| Human: OVCAR-3 | ATCC | HTB-161 |
| Human: OVCAR-4 | ExPASy | CVCL_1627 |
| Human: OVCAR-5 | ExPASy | CVCL_1628 |
| Human: CAVO3 | ATCC | HTB-75 |
| Human: COV362 | ExPASy | CVCL_2420 |
| Human: OV90 | Kind gift from Dr. Chip Landen, UVA | N/A |
| Human: OVSAHO | Kind gift from Dr. Chip Landen, UVA | N/A |
| Human: SKOV-3 | ATCC | HTB-77 |
| Human: Colo-205 | ATCC | CCL-222 |
| CHO-S cells | Thermo Scientific | R80007 |
| Mouse: ID8 | Kind gift from Dr. Chip Landen, UVA | N/A |
| Mouse: MC38 | Kind gift from Dr. Suzanne Ostrand-Rosenberg, UMBC | N/A |
| PDX cell line: V584 | Dr. Chip Landen, UVA | UVA MAPS core/Tumor bank |
| PDX cell line: V565 | Dr. Chip Landen, UVA | UVA MAPS core/Tumor bank |
| PDX cell line: 111 | Dr. Chip Landen, UVA | UVA MAPS core/Tumor bank |
| PDX cell line: 135R | Dr. Chip Landen, UVA | UVA MAPS core/Tumor bank |
| K562 cells | Kind gift from Dr. Golam Mohi, UVA | N/A |
| HEK293 | Kind gift from Dr. Sanchita Bhatnagar, UVA | N/A |
| Human: Colo-205-GFP stable | This Paper | Generated in laboratory of novel biologics, UVA |
| Mouse: MC38-Luc stable | This Paper | Generated in laboratory of novel biologics, UVA |
| Mouse: ID8-Luc stable | This Paper | Generated in laboratory of novel biologics, UVA |
| HEK | ATCC | CRL-1533 |
| Experimental Models: Organisms/Strains | ||
| Mouse: athymic Nude Foxn1nu/Foxn1+ | Envigo | RRID:MGI:5652489 |
| Mouse: C56BL/6 | Bhatnagar lab (UVA animal facility) | NA |
| Mouse: CD1(Crl:CD1(ICR) | Charles River | RRID:IMSR_CRL:22 |
| Mouse: C.B-17/IcrHsd-Prkdcscid | UVA MAPS core, Envigo, Dublin, VA | RRID:MGI:2160375 |
| Oligonucleotides | ||
| Primer FOLR1 Forward: GTCGACCCTGGAGGAAGAAT | This Paper | N/A |
| Primer FOLR1 Reverse: AGTCCAGTTCCAGCCCTTGT |
This Paper | N/A |
| Primer TRAIL-R2/DR5 Forward: GATGGTCAAGGTCGGTGATT |
This Paper | N/A |
| Primer TRAIL-R2/DR5 Reverse: TGGACTTCCATTTCCTGCTC |
This Paper | N/A |
| Primer GALNT3 Reverse: ACAGAGGTTCTAGCCAACCAT |
This Paper | N/A |
| Primer FUT3 Forward: CTGTCCCGCTGTTCAGAGATG |
This Paper | N/A |
| Primer FUT3 Reverse: AGGCGTGACTTAGGGTTGGA |
This Paper | N/A |
| Recombinant DNA | ||
| Full Length Lexatumumab IgG and scFv | This Paper | GeneArt, Thermo Fisher |
| Full Length Farletuzumab IgG and scFv | This Paper | GeneArt, Thermo Fisher |
| Full Length AMG-655 IgG and scFv | This Paper | GeneArt, Thermo Fisher |
| Full Length LK26 IgG and scFv | This Paper | GeneArt, Thermo Fisher |
| Full Length MD5–1 IgG and scFv | This Paper | GeneArt, Thermo Fisher |
| Full Length Recombinant DNA, huFOLR1-IgG4-Fc | This Paper | GeneArt, Thermo Fisher |
| Full Length Recombinant DNA, huDR5-IgG4-Fc | This Paper | GeneArt, Thermo Fisher |
| Full Length Idarucizumab IgG and scFv | GeneArt, Thermo Fisher | GeneArt, Thermo Fisher |
| pCDNA3.1+ | Thermo Fisher | V79020 |
| pET-28a | Addgene | 69864–3 |
| pTT5 | (Durocher and Butler, 2009) | Addgene (52326) |
| Software and Algorithms | ||
| Vector NTI | Thermo scientific | N/A |
| GraphPad Prism | GraphPad Software | www.graphpad.com |
| FlowJo | FlowJo, LLC | www.flowjo.com |
| FCS Express | De Novo Software | www.denovosoftware.com |
| Other | ||
| PowerUp SYBR Green Master mix | Thermo Fisher | A25742 |
| Superscript II | Invitrogen | 18064014 |
| Halt protease inhibitor | Thermo Fisher | 78430 |
| CHO CD efficient Feed B | Thermo Fisher | A1024001 |
| Tryptone Feed | Thermo Fisher | BP9726–2 |
| PEI transfection reagent | Thermo Fisher | BMS1003A |
| Matrigel | Corning | 354234 |
| Infusion | Takara BioScience | 638989 |
| IRDye 800CW | LI-COR | Cat#929–70020 |
| TMB Substrate Reagent Set | BD OptEIA | Cat # 555214 |
| 7 Aminoactinomycin-D (7-AAD) | Thermo Fisher | A1310 |
| CHO free style Media | Thermo Fisher | 12651014 |
| HiTrap MabSelect Sure column | GE | 11003493 |
| Protein-A resin | Thermo Fisher | P153142 |
| HisPur Ni-NTA resin | Thermo Fisher | 88221 |
| HiPure Plasmid Maxiprep kit | Invitrogen | K21007 |