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. 2019 Mar 7;14(3):e0210477. doi: 10.1371/journal.pone.0210477

Fig 1. Western blot analysis of cell lysates infected by the Baculo-P1-3CD-gp41 construct.

Fig 1

The proteins were separated by SDS-PAGE, electrotransferred to a nitrocellulose membrane, and probed using anti-VP2 MAb (MAb979) as the primary antibody (A). Hi-5 cells used for EV71 VLP large-scale production were infected with Baculo-P1-3CD-gp41 at MOI 5, and protein was analyzed using an anti-VP1 Mab produced in-house (B). Lane1: Baculo-P1-3CD-CMV-IE infected cell lysates; Lane2: Baculo-P1-3CD-lef3 infected cell lysates; Lane3: Baculo-P1-3CD-gp41 infected cell lysates; Lane4: Baculo-P1-3CD-chitinase infected cell lysates. The cells were harvested from 3 dpi.