Table 1. Comparison of VLP yield according to promoter type (units: mg/L).
Predicted MW (kDa) | Promoter type | ||||
---|---|---|---|---|---|
CMV-IE | Lef3 | gp41 | chitinase |
*gp41 extracellular |
|
Viral antigen expected in the EV71 virion (<40kDa) | 2.3 | 10 | 11.3 | 7 | 2.4 |
Total viral protein (including incomplete processed viral polypeptides) |
11.2 | 41.4 | 43.1 | 32.7 | 5.1 |
* Concentrated cell supernatant. VLP, virus-like particle; MW, molecular weight