Table 1. Mass spectroscopy results of proteins interacting exclusively with wtPOC5.
Scaffold software was used for the analysis of the identified proteins interacting with wtPOC5. Protein identification in wtPOC5 expressing cells, detected 85 candidates interacting with wtPOC5. Clustering proteins by biological function indicated: ciliary proteins (17 proteins); cell adhesion (7 proteins); cytoskeleton-associated protein (4 proteins); RNA processing (9 proteins); extracellular matrix (3 proteins); response to estrogen (1 protein); cell cycle and cytokinesis (1 protein). Most of the identified proteins interacting with POC5 are cytoskeletal proteins (As shown in the table) and those marked in bold were considered for further analysis. Other protein groups belong to cell cycle and cytokinesis, extracellular proteins, RNA processing, cell adhesion, and response to estrogen.
| Protein ID | Description | Score | Peptide Number |
| P15924 | Desmoplakin | 3984 | 119 |
| Q08554 | Desmocollin-1 | 581 | 12 |
| P68363 | Tubulin alpha-1B chain | 368 | 10 |
| P07355 | Annexin A2 | 221 | 9 |
| Q9NSK0-3 | Isoform 3 of Kinesin light chain 4 | 155 | 9 |
| F8VW92 | Tubulin beta chain | 251 | 7 |
| P47929 | Galectin-7 | 338 | 6 |
| Q9H223 | EH domain-containing protein 41 | 62 | 4 |
| Q6IB90 | Cystatin | 84 | 3 |
| Q9NZT1 | Calmodulin-like protein 5 | 243 | 3 |
| Q14574-2 | Isoform 3B of Desmocollin-3 | 63 | 3 |
| Q14008 | Cytoskeleton-associated protein 5 | 57 | 3 |
| Q9Y5P4-2 | Isoform 2 of Collagen type IV alpha-3-binding protein | 45 | 2 |
| P27482 | Calmodulin-like protein 3 | 144 | 2 |
| P08758 | Annexin A5 | 164 | 2 |
| Q08380 | Galectin-3-binding protein | 76 | 2 |
| Protein ID | Description | Score | Peptide Number |
| B4DF70 | cDNA FLJ60461; highly similar to Peroxiredoxin-2 (EC 1.11.1.15) | 88 | 2 |
| Q59FR8 | Galectin 3 | 2 | |
| Q9UHD8-5 | Isoform 5 of Septin-9 | 46 | 2 |
| J3KNF5 | Centrosomal protein of 290 kDa | 20 | 1 |
| Q8N6N5 | Tubulin; beta 2C | 85 | 1 |
| B4E0R6 | Importin-5 | 69 | 1 |
| Q86YS3-2 | Isoform 2 of Rab11 family-interacting protein 4 | 27 | 1 |
| A8MUB1 | Tubulin alpha-4A chain | 183 | 1 |
| A9X9K9 | Desmocollin 2 | 46 | 1 |
| r-Q5T802 | RUNX2 | 15 | 1 |
| Q13835 | Plakophilin-1 2 | 536 | 17 |
| F5GWP8 | Junction plakoglobin | 3102 | 16 |
| P25311 | Zinc-alpha-2-glycoprotein | 141 | 3 |
| Q9GZZ8 | Extracellular glycoprotein lacritin | 98 | 3 |
| E9PBV3 | Suprabasin | 190 | 3 |
| Q9Y5P4-2 | Isoform 2 of Collagen type IV alpha-3-binding protein | 45 | 2 |
| B4DT31 | Far upstream element-binding protein 1 | 151 | 9 |
| E9PIN3 | Nuclear RNA export factor 1 (Fragment) | 149 | 5 |
| O43776 | Asparagine—tRNA ligase; cytoplasmic | 94 | 4 |
| Q9Y2X3 | Nucleolar protein 58 | 113 | 4 |
| B8ZZD1 | U4/U6.U5 tri-snRNP-associated protein 2 | 43 | 2 |
| P35579 | Myosin-9 | 555 | 18 |
| P58107 | Epiplakin | 200 | 10 |
| Q8WVV4 | Protein POF1B | 242 | 6 |
| Q15149-9 | Isoform 9 of Plectin | 84 | 2 |