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. 2019 Mar 7;14(3):e0213385. doi: 10.1371/journal.pone.0213385

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© 2019 Perrucci et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Untreated G. duodenalis trophozoites showing normal structure and morphology (a). Trophozoite coronal section (c). A coronal view of a trophozoite demonstrates the nuclei (N), endoplasmic reticulum (ER), flagella (F), vacuoles (V), ventral disk (VD), lateral crest (LC) and ventrolateral flange (VLF). The same sections (b, d) of treated parasites show swelling trophozoites, with an increased size, and evident alterations of their cellular membrane and with a vacuolar degenerative pattern (x6,700). Note in the coronal section the severely damaged nuclei, nuclear membrane rupture, loss of the chromatin, flanges and ventral disk rupture (x6,700).