Figure 2.

Development of FSGS is abrogated by treatment with 2,4-diHB in Nphs2.Cre⁺;Coq6^flox/flox mutant mice. Kidney serial sections of 10.5-month-old mice with indicated genotypes were stained with Masson’s trichrome and analyzed to determine the severity of glomerular sclerosis. The Nphs2.Cre⁺;Coq6^flox/flox mutant mice show FSGS, whereas Nphs2.Cre⁺;Coq6^flox/flox mutant mice under treatment with 2,4-diHB displayed virtually no (<10% of glomeruli) abnormal histologic findings (n=3 animals in each group; each graph bar indicates an single animal, numbers inside the bar graphs indicate number of sclerosed glomeruli per total glomeruli counted in one section. Two-way ANOVA P values calculated using Tukey’s multiple comparisons test are shown in the figure. ****P<0.001.