Figure 1.

Inactivation of Sec63 and Xbp1 results in renal interstitial inflammation. (A) Hematoxylin and eosin staining and (B) Masson trichrome staining of renal cortex and medulla of the indicated genotypes at P70. WT and Sec63 mutant (SKO) kidneys do not show interstitial inflammation or fibrosis. Sec63 and Xbp1 double mutants (DKO) have extensive inflammatory infiltrates and areas of collagen deposition. Scale bar, 500 µm. (C) Quantitative assessment of body weight (body wt), percent two-kidney weight to body weight ratio (%KW/body wt), BUN, and serum creatinine (s-Cr). Kidney function indicated by BUN and s-Cr begins to decline by P49 in DKO kidneys. The colors correspond to the genotypes in (A). Results are shown as mean ± SEM (ANOVA among groups at each time point). ***P<0.001; **P<0.01; *P<0.05.