Figure 6.

Xbp1s is essential to suppress renal interstitial inflammation and fibrosis in kidneys with inactivation of Sec63. (A) Hematoxylin and eosin (H&E) staining of renal cortex and medulla of the indicated genotypes at P70 showing chronic kidney injury both DKOs and the triple knockout. Scale bar, 500 µm. (B) H&E staining of renal cortex and medulla of the indicated genotypes at P70 showing rescue of chronic kidney injury in both DKOs with transgenic re-expression of XBP1s. Scale bar, 500 µm. (C) Quantitative assessment of BUN and serum creatinine (s-Cr). The colors of the symbols correspond to the colors indicated for each of the genotype from (A and B). BUN and s-Cr indicate that Sec63-Xbp1 and Sec63-Ire1α as well as the triple knockout have impaired kidney function at P70. This impaired kidney function is resolved by re-expression of XBP1s on either DKO background. Results are shown as mean ± SEM (ANOVA). ***P<0.001; **P<0.01; *P<0.05. (D) Gene expression of F4/80 indicate that both DKO models have elevated levels that are resolved by re-expression of XBP1s. The color of the bars defines the genotypes on the basis of (A and B). Results are shown as mean ± SEM (ANOVA). n=3 mice for group; **P<0.01; *P<0.05.