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. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Cell Chem Biol. 2018 Dec 13;26(2):244–254.e4. doi: 10.1016/j.chembiol.2018.10.023

Figure. 5. Phagocytosis of live E. coli with macrophages overexpressing DRV1/GPR32: Real-time monitoring.

Figure. 5.

Human MΦ were transfected with human DRV1 plasmid or mock. MΦ were plated onto 8-well chamber slides (100,000 cells/well in PBS++) 48h after transfection. Imaging was then carried out 24h after re-plating. MΦ were incubated with test compounds (10 nM) or vehicle control for 15 min at 37°C, followed by addition of BacLight Green-labeled E. coli (E. coli: macrophage 50:1) to initiate phagocytosis. Fluorescent images were then recorded every 10 min for 60 min. Three separate experiments were carried out. In each experiment, 4 fields (40X) per condition (per well) were recorded. (A-E) (Left) Results are mean fluorescence of 4 fields/well from one representative experiment. (Right) % increase in phagocytosis above vehicle control in mock or GPR32 transfected MΦ at 10–60 min; mean±SEM from 3 separate donors. *P<0.05, vs Mock.